Genome analysis ofPseudomonas aeruginosaby field inversion gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Pulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
متن کاملPulsed field gel electrophoresis and genome size estimates.
Pulsed field gel electrophoresis (PFGE) is a quick and reliable procedure to resolve DNA molecules larger than 30 kb by applying an electric field that periodically changes direction. This technique can be used to estimate genome size of a microorganism, to reveal if a genome is circular or linear, to indicate the presence of megaplasmids, and to show if a strain contains only one or more chrom...
متن کاملPulsed field gel electrophoresis and investigations into mammalian genome organization.
Pulsed field gel (PFG) electrophoresis can resolve DNA molecules as large as several million base pairs (mbp) in size (Schwartz and Cantor, 1984; Carle and Olson, 1984; Gardiner et al. 1986; Carle et al. 1986; Chu et al. 1986; Clark et al. 1988; Anand, 1986; Gardiner and Patterson, 1988; Orbach et al. 1988). This is in contrast to conventional electrophoresis where the practical upper limit is ...
متن کاملDetermination of Wolbachia genome size by pulsed-field gel electrophoresis.
Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number of sites and were used for the determination of the genome sizes of wMelPop, ...
متن کاملGenome analysis of Legionella ssp. by orthogonal field alternation gel electrophoresis (OFAGE).
Various Legionella isolates from different sources and origins were analysed by orthogonal field alternation gel electrophoresis of NotI cleaved genomic DNA. The genome of L. pneumophila Philadelphia I, the original isolate of the epidemics in 1976, exhibits only five NotI fragments. Two virulent derivatives, derived from L. pneumophila Philadelphia I, which were obtained by prolonged passage o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1987
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1987.tb02635.x